FastQCFastQC Report
Fri 10 Feb 2017
ERR1147385.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1147385.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1323141
Sequences flagged as poor quality0
Sequence length125
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT49080.3709355238784075No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT29000.21917543179449506No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT27800.21010610358230905No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTTCATTAAT15440.11669202299679324No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAGGCCATGAT13300.10051838768506154No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA36550.056.245631
GGTATCA18850.054.3718571
TCAACGC48750.041.8477024
AACGCAG49200.041.4649546
CAACGCA50250.040.716885
ATCAACG50450.040.555463
GTACATG66300.036.7592661
TACATGG67650.036.002542
ACGCAGA57400.035.5413867
TATCAAC58950.035.0528452
ACATGGG68250.034.7714163
CGCAGAG60850.033.428568
CATGGGT21350.032.594184
GCAGAGT65900.031.1376489
CATGGGG32800.030.2827134
GGGTTAG4100.029.06721
AGAGTAC61400.027.22012310-11
CGTGCTA600.00128746624.7944458-59
CAGAGTA64300.023.81871810-11
TATACCG1751.7561635E-523.7909685
Produced by FastQC (version 0.11.5)