FastQCFastQC Report
Fri 10 Feb 2017
ERR1147384.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1147384.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2316696
Sequences flagged as poor quality0
Sequence length125
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT113110.48823842230486864No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT66840.2885143324803945No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT60280.260198144253713No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA88700.054.8449331
GGTATCA46400.053.7066731
AACGCAG113300.042.423226
TCAACGC118500.040.662014
CAACGCA121350.039.952145
ATCAACG120650.039.937413
TATCAAC126150.038.5787282
ACGCAGA133800.035.92347
CGCAGAG137350.035.1248448
TAACGCA1607.00129E-933.4614454
GTACATG132050.032.7769241
GCAGAGT147150.032.7451489
GGGTTAG8100.032.384531
TACATGG137000.031.4797382
ACATGGG135800.030.9700453
CATGGGG77350.029.2243944
ATGGGGG38150.028.067275
AGAGTAC140950.027.87596510-11
CATGGGT30550.025.8977854
CAGAGTA144700.025.52967510-11
Produced by FastQC (version 0.11.5)