FastQCFastQC Report
Fri 10 Feb 2017
ERR1147382.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1147382.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6308053
Sequences flagged as poor quality0
Sequence length125
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT233220.369717882839602No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT136710.21672297299975127No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT122310.19389501007680182No Hit
CTATAGAACTAGTACCGCAAGGGAAAGATGAAAGACTAATTAAAAGTAAG78630.12465018921052186No Hit
GATTAAACCTTGTACCTTTTGCATAATGAACTAACTAGAAAACTTCTAAC74750.11849932142294936No Hit
ACTAAAAGTATTGGAGAAAGAAATTCGTACATCTAGGAGCTATAGAACTA73280.11616896687456493No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA227100.047.3629881
AACGCAG256600.041.863346
GGTATCA128450.041.6601871
GTACATG335250.037.2386781
TCAACGC288250.037.0603684
CAACGCA293500.036.458255
ACGCAGA295200.036.3490377
ACATGGG335500.036.3086553
ATCAACG296850.036.026773
CGCAGAG298850.036.0046048
CATGGGT136000.035.8630874
TACATGG357850.034.83422
TATCAAC317250.034.073182
GCAGAGT331650.032.569319
CATGGGG136350.032.542914
ATGGGTG75600.029.9761565
ATGGGGG54600.028.1060525
AGAGTAC327700.027.54387310-11
CAGAGTA339500.023.91473810-11
ATGGGTA50250.023.7920955
Produced by FastQC (version 0.11.5)