FastQCFastQC Report
Fri 10 Feb 2017
ERR1147381.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1147381.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5112102
Sequences flagged as poor quality0
Sequence length125
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT119620.23399376616507261No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT67500.13203961892779134No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT64250.125682155794231No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA119700.049.053441
GGTATCA62850.043.914011
AACGCAG141850.040.672446
TCAACGC147050.039.0319374
CAACGCA150000.038.264315
GTACATG208350.037.852431
ATCAACG154100.037.400643
ACATGGG210600.036.206583
ACGCAGA165600.034.8752067
CGCAGAG167950.034.2809758
TACATGG227700.034.032412
TATCAAC176450.032.8450282
CATGGGT68000.031.4009934
CATGGGG101250.031.2517574
GCAGAGT191900.030.2815049
ATGGGGG48850.027.0300165
AGAGTAC181450.026.86270910-11
TGGGGGG39550.023.310046
ATGGGTA33600.022.4813465
GAGTACT88500.022.11110712-13
Produced by FastQC (version 0.11.5)