FastQCFastQC Report
Fri 10 Feb 2017
ERR1147379.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1147379.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6205543
Sequences flagged as poor quality0
Sequence length125
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT161440.2601545102499491No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT102030.16441752156096573No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT84860.1367487099839611No Hit
GATTAAACCTTGTACCTTTTGCATAATGAACTAACTAGAAAACTTCTAAC63120.10171551466165007No Hit
ACTAAAAGTATTGGAGAAAGAAATTCGTACATCTAGGAGCTATAGAACTA62870.10131264903006877No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA185200.048.1174771
GGTATCA100650.046.6083371
AACGCAG217300.040.732266
TCAACGC228700.038.3377574
CAACGCA234900.037.401835
ATCAACG238300.036.8432273
CATGGGT131850.035.6854934
GTACATG305600.035.577391
ACGCAGA250400.035.3479277
CGCAGAG251500.035.1696748
ACATGGG299950.034.9820333
TATCAAC257000.034.3268242
TACATGG324150.033.224932
GCAGAGT278850.031.826849
CATGGGG114600.029.4821454
GCGTATC3150.028.3846361
ATGGGTG68950.027.692755
AGAGTAC269950.027.19112610-11
ATGGGTA53100.025.9889555
ATGGGGG47300.025.2772755
Produced by FastQC (version 0.11.5)