FastQCFastQC Report
Fri 10 Feb 2017
ERR1147378.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1147378.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2753337
Sequences flagged as poor quality0
Sequence length125
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT103470.3757985310189054No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT66460.2413798238283218No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT56150.20393435311405758No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA45750.054.4354361
GTATCAA81550.048.5841031
AACGCAG102100.038.5089656
TCAACGC105500.036.873254
ATCAACG107200.036.343993
CAACGCA108350.035.958255
TATCAAC119400.032.912412
ACGCAGA121000.032.4939277
GTACATG131100.032.4483071
ACATGGG132500.031.6489543
CGCAGAG124950.031.5619168
TACATGG135900.031.2384992
GCAGAGT137400.029.0483789
CATGGGG70350.028.916664
CATGGGT37550.028.354974
GGGTTAG10000.028.0023421
ATGGGGG33300.026.9723535
AGAGTAC131150.024.67270310-11
TAGGTCG2001.832188E-623.792835
CAGAGTA136300.023.71864310-11
Produced by FastQC (version 0.11.5)