FastQCFastQC Report
Fri 10 Feb 2017
ERR1147376.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1147376.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2354156
Sequences flagged as poor quality0
Sequence length125
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT111080.47184638571105736No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT67400.28630218218333875No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT61630.26179233661660484No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA92400.050.906391
GGTATCA51550.044.293231
AACGCAG108900.042.824776
TCAACGC118050.039.5558364
ATCAACG121150.038.5436743
CAACGCA121400.038.4643025
ACGCAGA127900.036.4637
TATCAAC129700.036.2891772
CGCAGAG131000.035.781778
GCAGAGT142050.032.998329
TATAACG2203.6379788E-1232.4973952
GTACATG123750.032.1809231
TACATGG128450.030.890872
ACATGGG126850.030.715513
CATGGGG65400.027.6504844
AGAGTAC134650.027.63298610-11
CATGGGT34650.026.0943974
CAGAGTA138300.025.22625210-11
GAGTACT71700.024.43278712-13
TAACGCA2201.5439036E-724.334744
Produced by FastQC (version 0.11.5)