FastQCFastQC Report
Fri 10 Feb 2017
ERR1147375.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1147375.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2892187
Sequences flagged as poor quality0
Sequence length125
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT87050.3009833043299067No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT51060.17654460102337782No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT47010.16254135711141776No Hit
CTATAGAACTAGTACCGCAAGGGAAAGATGAAAGACTAATTAAAAGTAAG32490.11233713449372396No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA74250.051.2058221
GGTATCA39150.047.035081
AACGCAG86150.043.0792056
TCAACGC88000.042.241154
CAACGCA90000.041.2363745
ATCAACG92000.040.4045753
ACGCAGA100250.036.8422057
CGCAGAG103850.035.8514068
TATCAAC106650.035.1849332
GTACATG112450.034.4998051
ACATGGG115400.032.5208553
TACATGG120300.032.1333582
GCAGAGT117700.031.7842949
GGGTTAG8900.030.8008691
CATGGGT31350.030.1646274
CATGGGG57450.028.3660534
ATGGGGG32800.026.4738675
AGAGTAC111900.026.2298310-11
TATCCTA16100.024.750695
GAGTACT56600.024.53628512-13
Produced by FastQC (version 0.11.5)