FastQCFastQC Report
Fri 10 Feb 2017
ERR1147374.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1147374.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2738311
Sequences flagged as poor quality0
Sequence length125
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT94400.34473805203280417No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT54450.19884520056341298No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT53400.19501072011177692No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTTCATTAAT30810.11251461210943534No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA81250.053.3901861
GGTATCA44000.048.211511
AACGCAG101450.042.1576356
TCAACGC105450.040.614894
ATCAACG107850.039.9868553
CAACGCA107800.039.7846875
ACGCAGA117600.036.4187137
CGCAGAG120950.035.360838
TATCAAC125050.034.478962
GTACATG131300.033.76461
ACATGGG135150.032.173683
TACATGG136200.032.1373062
GCAGAGT133300.032.040099
CATGGGG63700.028.1077774
CATGGGT44250.027.557514
AGAGTAC127650.027.35373910-11
ATGGGGG30850.026.6086795
CAGAGTA132000.023.97381210-11
TATACCG1802.1830385E-523.1326265
AGTACTT70400.022.4754512-13
Produced by FastQC (version 0.11.5)