FastQCFastQC Report
Fri 10 Feb 2017
ERR1147373.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1147373.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4296840
Sequences flagged as poor quality0
Sequence length125
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT94010.21878869122424852No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT54250.12625557386358346No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT52460.12208972174900624No Hit
ATCTATAACTTTATAGATGCAACACTGTTAGTATGAGTAACAAGAATTCC50070.11652749462395622No Hit
GATTAAACCTTGTACCTTTTGCATAATGAACTAACTAGAAAACTTCTAAC43980.10235428826765718No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA100200.042.713641
AACGCAG109600.038.1543436
TCAACGC117800.035.4984364
GGTATCA59000.035.2600751
CAACGCA121150.034.6150445
GTACATG143600.034.245961
ATCAACG124050.033.8058243
ACGCAGA126300.033.2035837
ACATGGG142150.033.1837543
CGCAGAG131450.031.9027218
TACATGG153700.031.4306282
TATCAAC138950.030.523022
CATGGGG66200.028.4839654
GCAGAGT150350.027.9714629
CATGGGT45200.026.9783024
AGAGTAC143450.025.6263710-11
ATGGGGG27950.025.3258675
TAATACT41850.025.158054
CTTAATA28500.024.0358242
TATCCTA25300.022.5709535
Produced by FastQC (version 0.11.5)