FastQCFastQC Report
Fri 10 Feb 2017
ERR1147372.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1147372.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9244781
Sequences flagged as poor quality0
Sequence length125
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT254060.27481451426485926No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT144390.15618541964379687No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT137020.14821335410757702No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTTCATTAAT94360.10206839945694765No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA229800.057.040071
GGTATCA113000.051.3262141
AACGCAG295400.044.0999266
TCAACGC297400.043.683354
CAACGCA304000.042.754535
ATCAACG313350.041.4408153
ACGCAGA337250.038.574577
CGCAGAG348800.037.229018
TATCAAC352700.037.2208482
GCAGAGT388050.033.632049
GTACATG368500.032.2061351
ACATGGG368600.031.0010433
TACATGG383150.030.7805562
CATGGGT115650.030.6038154
AGAGTAC369950.028.72527910-11
CATGGGG181050.026.119984
GAGTACT165050.024.29118212-13
CAGAGTA392150.024.05776210-11
AGTACTT195600.022.93014312-13
TATCCTA41950.022.8295925
Produced by FastQC (version 0.11.5)