FastQCFastQC Report
Fri 10 Feb 2017
ERR1147371.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1147371.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4825272
Sequences flagged as poor quality0
Sequence length125
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT169680.351648570277489No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT94050.1949112920473706No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT89380.18523308116102055No Hit
ATCTATAACTTTATAGATGCAACACTGTTAGTATGAGTAACAAGAATTCC50620.1049060032263466No Hit
GATTAAACCTTGTACCTTTTGCATAATGAACTAACTAGAAAACTTCTAAC49720.1030408233981421No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA139350.048.9799421
AACGCAG160700.042.1590236
GGTATCA76800.041.7581061
TCAACGC176150.038.4612854
CAACGCA180500.037.468475
ATCAACG182350.037.120963
ACGCAGA185900.036.4120867
CGCAGAG191150.035.443138
GTACATG199150.035.050671
ACATGGG199850.033.989493
TATCAAC203350.033.7448842
TACATGG214600.032.3367162
GCAGAGT212450.032.0016379
CATGGGT60500.030.0849134
CATGGGG97200.028.5780964
AGAGTAC204350.027.89974210-11
TATCCTA26800.025.5238255
ATGGGGG39950.024.8646535
TAATACT42350.024.2982714
GAGTACT111850.024.09044812-13
Produced by FastQC (version 0.11.5)