FastQCFastQC Report
Fri 10 Feb 2017
ERR1147368.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1147368.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4843392
Sequences flagged as poor quality0
Sequence length125
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT213780.4413848806786648No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT118770.2452207048283517No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT115970.23943963238986232No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA162450.048.606561
AACGCAG189350.041.6551976
GGTATCA93300.037.9405441
TCAACGC208550.037.5350234
CAACGCA214550.036.596235
ATCAACG213850.036.5769463
ACGCAGA222350.035.44627
GTACATG225300.034.9414021
CGCAGAG227050.034.8172458
TATCAAC233450.033.7789272
TACATGG234300.033.503862
ACATGGG229900.033.4024433
GCAGAGT248900.031.928069
CATGGGG122150.029.5100634
ATGGGGG51150.029.0726975
CATGGGT59450.028.5156974
AGAGTAC237800.027.92787410-11
ATGGGTA29150.024.6909485
CAGAGTA251900.022.92883710-11
AGTACTT150700.022.77474412-13
Produced by FastQC (version 0.11.5)