FastQCFastQC Report
Fri 10 Feb 2017
ERR1147367.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1147367.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3827795
Sequences flagged as poor quality0
Sequence length125
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT192150.5019861303962203No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT105060.2744661090784642No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT103780.27112214734592627No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA148500.048.8030431
GGTATCA85100.044.261621
AACGCAG174550.041.2987256
TCAACGC192500.037.2005734
ATCAACG194850.036.7519153
CAACGCA196000.036.6880075
ACGCAGA202700.035.5046737
GTACATG202700.035.2537421
TATCAAC209800.034.3482
CGCAGAG209850.034.1815878
TACATGG210200.033.8576472
ACATGGG208850.033.4339373
GCAGAGT226550.031.8194339
ATGGGGG55800.029.9520155
CATGGGG119650.029.8258254
TGGGGGG47400.027.9821076
CATGGGT49200.027.925494
AGAGTAC219900.026.93940510-11
ATGGGTA23600.025.706455
CAGAGTA224200.024.56570610-11
Produced by FastQC (version 0.11.5)