FastQCFastQC Report
Fri 10 Feb 2017
ERR1147366.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1147366.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4302991
Sequences flagged as poor quality0
Sequence length125
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT155840.3621666882408074No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT87890.20425327405983418No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT85760.19930322884709728No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTTCATTAAT44240.10281220667205672No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAGGCCATGAT43990.10223121544990449No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA143350.052.5172421
GGTATCA76200.043.5708921
AACGCAG174150.043.1035166
TCAACGC184950.040.4578824
CAACGCA188050.039.8541955
ATCAACG189200.039.5490763
ACGCAGA199450.037.6358877
CGCAGAG203500.036.9453248
GTACATG226150.036.188471
TATCAAC211750.035.5873342
CATGGGT85000.035.5485274
ACATGGG226550.035.4180533
TACATGG237550.034.2549972
GCAGAGT224350.033.670889
ATGGGTA36150.028.6297635
CATGGGG93550.028.6118264
AGAGTAC217500.028.09955410-11
ATGGGTG46200.025.7492665
ATGGGGG37900.023.8550975
CAGAGTA227850.023.8340910-11
Produced by FastQC (version 0.11.5)