FastQCFastQC Report
Fri 10 Feb 2017
ERR1147365.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1147365.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5028690
Sequences flagged as poor quality0
Sequence length125
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT106200.211188202096371No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT60260.11983240167916494No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT54480.10833835452175418No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA121650.048.505011
GGTATCA63300.042.3713531
AACGCAG141400.040.3838586
TCAACGC147950.038.716614
CAACGCA151650.037.61515
ATCAACG155600.036.813133
ACGCAGA163150.035.1095477
CGCAGAG167200.034.2235348
GTACATG190400.033.338511
TATCAAC176750.033.0264552
ACATGGG186000.032.6191943
GCAGAGT190250.030.2959869
TACATGG207850.030.0335432
CATGGGG96800.029.8639284
CATGGGT51100.028.0531854
ATGGGGG48800.028.0345785
AGAGTAC181100.027.08059910-11
GGGTTAG10900.026.7937241
TGGGGGG39750.024.3913886
TAATACT46200.022.7885824
Produced by FastQC (version 0.11.5)