FastQCFastQC Report
Fri 10 Feb 2017
ERR1147364.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1147364.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2688298
Sequences flagged as poor quality0
Sequence length125
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT106460.39601264443153256No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT60540.2251982481108865No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT58590.21794458798838523No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA86050.051.9999731
GGTATCA46800.044.3044621
AACGCAG105700.041.9768526
TCAACGC113450.038.952054
CAACGCA116050.038.1818665
ATCAACG116450.038.0507133
ACGCAGA124750.035.662117
CGCAGAG128850.034.8043028
TATCAAC129750.034.4211432
GTACATG133450.034.1998521
TACATGG139050.032.8041762
ACATGGG138550.032.5822643
GCAGAGT140650.031.9689279
CATGGGT36750.030.9116384
CATGGGG70850.028.038364
AGAGTAC133350.027.67545510-11
TATAACG2506.9485395E-1026.2013722
GGGTACC16550.025.1562587
TATCACG1701.3908477E-524.5200022
ATGGGTA20400.024.4903565
Produced by FastQC (version 0.11.5)