FastQCFastQC Report
Fri 10 Feb 2017
ERR1147363.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1147363.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2576005
Sequences flagged as poor quality0
Sequence length125
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT95970.3725536247018154No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT58150.22573713948536592No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT54090.20997630051183908No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA91550.040.5738871
AACGCAG105500.034.9563876
GGTATCA58650.033.242731
TCAACGC112250.032.8543324
CAACGCA114250.032.1750765
ATCAACG115050.032.0030483
GTACATG125550.030.2984871
ACGCAGA122600.030.1292577
CGCAGAG127500.028.971358
ACATGGG129150.028.6472723
TATCAAC128900.028.6143842
TACATGG134750.028.1238652
CATGGGG61650.027.0154534
GCAGAGT137450.026.8741179
CATGGGT32400.024.9678554
AGAGTAC128100.024.2618810-11
AGTACTT69600.022.90408112-13
GAGTACT61950.022.03579312-13
ATGGGGG25150.021.5224025
CAGAGTA134150.020.97286610-11
Produced by FastQC (version 0.11.5)