FastQCFastQC Report
Fri 10 Feb 2017
ERR1147360.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1147360.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4448523
Sequences flagged as poor quality0
Sequence length125
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT202310.45478015961702345No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT110600.2486218459475201No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT107030.24059671041377104No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA161650.050.4473881
AACGCAG186550.043.555266
GGTATCA90150.042.848691
TCAACGC205050.039.5095834
ATCAACG208150.038.806863
CAACGCA209500.038.670365
GTACATG228600.037.9937021
ACGCAGA219000.037.074367
ACATGGG236500.036.1670343
CGCAGAG225200.036.1329048
TACATGG240400.036.1027572
TATCAAC228550.035.6289142
CATGGGG127600.033.7033274
GCAGAGT244550.033.419839
ATGGGGG59450.032.8176235
CATGGGT61100.032.3207934
AGAGTAC233950.028.32352410-11
TGGGGGG50200.025.8308036
AGTACTT135450.024.9433312-13
CAGAGTA240850.024.84485610-11
Produced by FastQC (version 0.11.5)