FastQCFastQC Report
Fri 10 Feb 2017
ERR1147359.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1147359.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4753353
Sequences flagged as poor quality0
Sequence length125
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT155860.3278948565360073No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT91070.1915910726596573No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT81490.17143687834671653No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA141700.046.270161
GGTATCA82850.041.079181
AACGCAG161400.040.171976
TCAACGC177800.036.4331134
CAACGCA182100.035.703475
GTACATG212200.035.6727451
ATCAACG184500.035.110073
ACGCAGA188400.034.3832667
ACATGGG215450.034.3182263
TACATGG222800.033.872462
CGCAGAG193050.033.7399528
TATCAAC203000.032.334852
GCAGAGT216500.030.3052469
CATGGGG111250.030.0494484
CATGGGT61950.029.093874
ATGGGGG51900.028.9970175
AGAGTAC208600.026.59100310-11
CAGAGTA216150.023.06157510-11
ATGGGTA29850.022.5182325
GAGTACT104450.022.35277212-13
Produced by FastQC (version 0.11.5)