FastQCFastQC Report
Fri 10 Feb 2017
ERR1147358.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1147358.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6235141
Sequences flagged as poor quality0
Sequence length125
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT151970.24373145691492784No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT85920.1377996103055248No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT77930.12498514468237366No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA167500.044.2960931
AACGCAG183450.039.523946
GGTATCA94850.036.881761
TCAACGC200100.036.1460384
CAACGCA204150.035.45815
ATCAACG207450.034.9513933
GTACATG253700.034.3899461
ACGCAGA212100.034.1851357
ACATGGG251650.033.8706443
CGCAGAG219050.033.2091268
TACATGG264900.032.7354432
CATGGGT76650.030.6520254
TATCAAC243300.029.9625032
GCAGAGT246200.029.8851819
CATGGGG118950.027.5025184
AGAGTAC232550.026.51106810-11
CAGAGTA248050.021.88103710-11
GAGTACT114100.021.08662812-13
ATGGGTA41950.020.9847585
AGTACTT134800.019.72385812-13
Produced by FastQC (version 0.11.5)