FastQCFastQC Report
Fri 10 Feb 2017
ERR1147356.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1147356.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4041193
Sequences flagged as poor quality0
Sequence length125
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT118060.2921414542685786No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT75100.18583621222742888No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT65210.16136324100333738No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA111650.047.0350761
GGTATCA62650.046.9062351
AACGCAG132500.039.008256
TCAACGC138700.037.3074344
CAACGCA143400.036.0017135
ATCAACG146250.035.3814773
GTACATG177200.035.2198641
ACGCAGA153200.033.81527
ACATGGG180450.033.7516983
TACATGG189050.032.9262962
CGCAGAG157850.032.819068
TATCAAC163950.031.8633482
CATGGGT54750.030.309074
GCAGAGT174150.029.7814319
CATGGGG88050.028.7086074
ATGGGGG43750.027.5975515
AGAGTAC168600.026.01698910-11
TGGGGGG36050.024.0879696
CAGAGTA176900.022.93026710-11
GAGTACT80650.021.38681812-13
Produced by FastQC (version 0.11.5)