FastQCFastQC Report
Fri 10 Feb 2017
ERR1147355.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1147355.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4660033
Sequences flagged as poor quality0
Sequence length125
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT145000.3111565948138135No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT82980.17806740853551897No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT74730.16036367124438816No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA130050.054.043731
GGTATCA67450.049.493471
TCAACGC164250.042.1534
AACGCAG165400.041.752036
CAACGCA170100.040.6683245
ATCAACG171800.040.26593
ACGCAGA191150.036.127577
TATCAAC195150.035.805072
CGCAGAG196400.035.1618428
GTACATG217850.034.8621141
ACATGGG223100.033.4066053
GCAGAGT215500.032.404239
CATGGGT71800.032.3916554
TACATGG234300.032.0850072
CATGGGG102850.027.702064
AGAGTAC205850.026.69942910-11
ATGGGTG40400.025.3237485
TATCACG2451.603803E-824.3135852
ATGGGGG49650.024.199875
CAGAGTA216200.023.8530710-11
Produced by FastQC (version 0.11.5)