FastQCFastQC Report
Fri 10 Feb 2017
ERR1147352.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1147352.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1068040
Sequences flagged as poor quality0
Sequence length125
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT30440.28500805213287894No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT20650.19334481854612187No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT15830.1482154226433467No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTTCATTAAT12490.11694318564847758No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA35400.044.0718881
GGTATCA22050.044.0192451
AACGCAG43150.035.419816
TCAACGC44350.034.8637124
ATCAACG44900.034.5691033
CAACGCA45350.033.8326765
TATCAAC50400.030.8096642
ACGCAGA50500.030.3824147
CGCAGAG52650.029.0287748
GCAGAGT56550.026.9216279
GTACATG54100.026.636781
TACATGG55850.026.3118112
CATGGGT11750.025.306154
ACATGGG56500.025.261363
ACCGTAT1250.001638341723.787788
ATGGGGG13450.023.434065
CATGGGG30250.023.1980024
ATGGGTA7400.022.5019555
CAGAGTA55600.022.35452510-11
AGAGTAC52800.021.73788810-11
Produced by FastQC (version 0.11.5)