FastQCFastQC Report
Fri 10 Feb 2017
ERR1147351.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1147351.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4236517
Sequences flagged as poor quality0
Sequence length125
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT194810.45983528450375627No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT108090.2551388322057955No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT104770.24730220603387168No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA148650.050.2913171
GGTATCA83400.045.1048971
AACGCAG180000.041.274866
TCAACGC194400.038.0644724
ATCAACG196250.037.766273
CAACGCA197550.037.5779655
GTACATG217850.036.0950471
ACGCAGA211950.035.0810247
TATCAAC215950.034.7530252
TACATGG228100.034.338062
ACATGGG224550.034.3310933
CGCAGAG218950.033.959468
CATGGGG122750.032.467514
GGGTTAG16000.032.4160161
ATGGGGG62850.032.2734075
GCAGAGT237350.031.4521399
TGGGGGG55000.028.768336
CATGGGT57250.027.94944
AGAGTAC227650.026.93932310-11
GTTAGAG18600.026.5436633
Produced by FastQC (version 0.11.5)