FastQCFastQC Report
Fri 10 Feb 2017
ERR1147350.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1147350.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3849360
Sequences flagged as poor quality0
Sequence length125
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT97920.2543799488746181No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT55750.14482927032026105No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT50990.1324635783610782No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA96250.051.193751
GGTATCA51400.043.2372361
AACGCAG118800.040.8524486
TCAACGC120350.040.2274634
CAACGCA123350.039.2973065
ATCAACG126400.038.2549633
ACGCAGA137100.035.5730177
TATCAAC143400.034.2567252
CGCAGAG144000.033.9097828
GTACATG162000.033.6525761
ACATGGG166550.032.3182833
TACATGG172300.031.6556512
GCAGAGT160400.030.5910179
CATGGGG88400.027.5179064
CATGGGT46000.026.2471734
AGAGTAC150150.025.98500610-11
ATGGGGG44150.025.3262845
CAGAGTA159100.022.52325610-11
GAGTACT67200.022.34788312-13
TGGGGGG35350.021.704246
Produced by FastQC (version 0.11.5)