FastQCFastQC Report
Fri 10 Feb 2017
ERR1147349.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1147349.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3245860
Sequences flagged as poor quality0
Sequence length125
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT93040.28664206096381234No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT50820.15656867517391385No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT50700.15619897346157874No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA90200.048.8228951
AACGCAG102300.042.0957576
TCAACGC112850.038.265774
CAACGCA115450.037.3524785
GGTATCA52050.037.2090231
ATCAACG116100.037.143363
ACGCAGA118700.036.3798837
CGCAGAG122100.035.512998
GTACATG133400.034.1289671
TATCAAC133500.032.9174162
ACATGGG134700.032.8534283
TACATGG140750.032.4487232
GCAGAGT136700.031.76369
CATGGGT35650.029.3648994
AGAGTAC130850.027.706110-11
CATGGGG69450.026.7213084
CAGAGTA138350.022.7217210-11
AGTACTT79950.021.4636112-13
GAGTACT68550.020.95487412-13
GGGTTAG7750.020.7609731
Produced by FastQC (version 0.11.5)