FastQCFastQC Report
Fri 10 Feb 2017
ERR1147348.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1147348.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3962411
Sequences flagged as poor quality0
Sequence length125
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT100530.25370916848353187No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT55600.14031860904888463No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT51170.12913854721279544No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA102650.047.848921
AACGCAG117800.040.749946
GGTATCA57050.040.435161
TCAACGC125250.038.326094
CAACGCA129150.037.122685
ATCAACG130350.036.917833
GTACATG164700.036.3727651
ACGCAGA136150.035.2577487
ACATGGG166700.034.9337963
CGCAGAG141550.033.8286558
TACATGG177700.033.354472
TATCAAC148950.032.5938382
CATGGGT48500.032.378814
GCAGAGT160800.030.2967879
CATGGGG81350.028.5902464
AGAGTAC149700.026.30479210-11
ATGGGGG35150.025.891955
CAGAGTA160200.022.2600110-11
ATGGGTG30600.021.5774565
GAGTACT73100.021.1163412-13
Produced by FastQC (version 0.11.5)