FastQCFastQC Report
Fri 10 Feb 2017
ERR1147347.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1147347.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2936713
Sequences flagged as poor quality0
Sequence length125
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT145960.4970182649785662No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT81120.2762271968694251No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT81120.2762271968694251No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT32880.11196191115713384No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTTCATTAAT30580.10413002564431731No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA103950.054.2049641
GGTATCA56900.049.460831
AACGCAG129850.042.9705546
TCAACGC137500.040.6230934
ATCAACG140400.039.7416463
CAACGCA141250.039.5446055
TATCAAC153200.036.8302232
ACGCAGA152450.036.561357
CGCAGAG155750.035.7866978
GTACATG157000.035.0157131
TACATGG165400.033.1770062
ACATGGG162400.032.9293943
GCAGAGT171100.032.5761459
CATGGGT42250.030.6930374
CATGGGG87400.029.5384944
GGGTTAG7700.028.6511921
ATGGGGG43850.027.5382615
AGAGTAC164100.027.13271910-11
CAGAGTA167250.025.25237810-11
GAGTACT85650.024.65534212-13
Produced by FastQC (version 0.11.5)