FastQCFastQC Report
Fri 10 Feb 2017
ERR1147346.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1147346.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1051460
Sequences flagged as poor quality0
Sequence length125
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT38940.37034219085842546No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT26170.24889201681471476No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT21620.2056188537842619No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTTCATTAAT12400.11793125748958591No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA21950.053.2041851
GTATCAA36400.045.6695861
AACGCAG45950.037.0255936
TCAACGC46700.035.7940564
CAACGCA47600.035.117285
ATCAACG47600.034.8673363
GGGTTAG3150.032.1560441
ACGCAGA52800.032.109427
TATCAAC52500.032.086122
CGCAGAG55350.030.9525438
GTACATG51850.030.222561
TACATGG54300.029.049332
GCAGAGT59150.028.863489
ACATGGG54150.028.1230873
CATGGGG27050.026.6096784
GGTTAGA3650.026.092592
CAGAGTA58000.024.76912510-11
ATGGGGG13300.024.5998555
AGAGTAC56400.024.20611610-11
GAGTACT29050.023.34426712-13
Produced by FastQC (version 0.11.5)