FastQCFastQC Report
Fri 10 Feb 2017
ERR1147345.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1147345.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2239420
Sequences flagged as poor quality0
Sequence length125
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT85530.38192924953782675No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT47010.20992042582454384No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT42780.1910316063980852No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA88150.050.022661
AACGCAG110300.039.7980546
GGTATCA50350.037.8710981
TCAACGC116000.037.4835174
ATCAACG116850.037.2617533
CAACGCA119150.036.4925585
TATCAAC125250.035.186682
ACGCAGA126950.034.5783777
CGCAGAG130750.033.618918
GTACATG135400.031.2461871
GCAGAGT141950.031.0082539
ACATGGG136750.030.5344943
TACATGG139200.030.3341122
CATGGGG71150.027.2535864
AGAGTAC136150.026.78081710-11
CATGGGT34500.025.6890544
ATGGGGG28000.023.7925475
AGTACTT66200.023.72066912-13
ATGGGAG22900.022.857475
CAGAGTA140600.022.400710-11
Produced by FastQC (version 0.11.5)