FastQCFastQC Report
Fri 10 Feb 2017
ERR1147344.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1147344.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3153282
Sequences flagged as poor quality0
Sequence length125
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT156870.4974816714775272No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT84900.26924328366444866No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT81580.25871457104058565No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA153650.048.493971
GGTATCA89650.041.0246731
AACGCAG184600.040.499416
TCAACGC202500.036.860724
CAACGCA205250.036.395835
ATCAACG204400.036.343493
ATGGGGG59000.035.4842645
TATCAAC211450.035.2157252
ACGCAGA213450.035.025497
GTACATG230950.034.5083161
CGCAGAG218850.034.215618
TACATGG237750.033.4749762
CATGGGG130350.033.3086434
ACATGGG237150.033.155343
GCAGAGT233600.032.258859
TGGGGGG46750.030.4061496
CATGGGT45800.030.3875374
AGAGTAC225700.026.90539210-11
ATATGTA14950.025.5181431
AGTACTT102000.024.54861612-13
Produced by FastQC (version 0.11.5)