FastQCFastQC Report
Fri 10 Feb 2017
ERR1147343.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1147343.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2469110
Sequences flagged as poor quality0
Sequence length125
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT49610.20092259964116627No Hit
GTACATGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGC33650.13628392416700755No Hit
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG28060.11364418758176022No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT25010.10129155849678628No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT24830.10056255087865669No Hit
GTACATGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTG24780.10036004876250956No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA61200.057.846521
GGTATCA31400.050.6785131
TCAACGC82200.042.329774
CAACGCA84400.041.226395
ATCAACG84800.040.961793
AACGCAG87000.040.0627026
GTACATG143350.038.7905731
ATGGGGG54500.037.651695
TATCAAC93100.037.6150442
TACATGG148100.037.399162
ACATGGG148300.036.577633
TGGGGGG41800.036.2848856
CATGGGG86450.036.05194
ACGCAGA101600.034.422757
CGCAGAG106150.033.003298
GCAGAGT117550.029.9544229
GTAAGTA18850.029.6995872
CATGGGT34700.027.9395894
ATATGTA10900.026.2456281
TATGTAA11800.026.2455232
Produced by FastQC (version 0.11.5)