FastQCFastQC Report
Fri 10 Feb 2017
ERR1147342.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1147342.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3714225
Sequences flagged as poor quality0
Sequence length125
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT106000.28538928040169886No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT58510.157529498078334No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT55840.15034091903425345No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA114900.056.322871
GGTATCA59400.044.14091
AACGCAG152800.041.6890346
TCAACGC155050.041.2758674
CAACGCA158850.040.176145
ATCAACG159750.039.9497953
TATCAAC166100.038.6837122
GTACATG222800.036.909561
ACGCAGA178700.035.613527
ACATGGG230200.034.9322933
CGCAGAG182850.034.8377578
TACATGG234700.034.8110662
ATGGGGG64900.033.633895
GCAGAGT199150.032.0460939
CATGGGG122250.031.3323294
CATGGGT59800.031.0319524
TGGGGGG54500.030.1209226
ATATGTA18050.029.3824331
TATGTAA20150.028.3708632
AGAGTAC194100.026.65934210-11
Produced by FastQC (version 0.11.5)