FastQCFastQC Report
Fri 10 Feb 2017
ERR1147341.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1147341.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6765764
Sequences flagged as poor quality0
Sequence length125
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG68850.10176234346926673No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA151200.049.831761
TCAACGC189400.039.1952174
CAACGCA193200.038.455095
AACGCAG194500.038.3203936
ATCAACG198050.037.513373
GGTATCA83650.035.2736321
TATCAAC218400.034.5302282
ACGCAGA222550.033.437077
GTACATG320550.033.119141
CGCAGAG230700.032.3073968
ACATGGG322900.031.8511733
TACATGG337800.031.1598452
ATGGGGG78350.030.216395
CATGGGG152500.029.7613894
GCAGAGT260750.028.880719
TGGGGGG60100.026.8221326
ATATGTA29700.026.4928341
CATGGGT89000.026.3332654
GTAAGTA46200.025.0137122
TATGTAA34700.024.8918342
Produced by FastQC (version 0.11.5)