FastQCFastQC Report
Fri 10 Feb 2017
ERR1147340.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1147340.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5715744
Sequences flagged as poor quality0
Sequence length125
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT100470.1757776415458775No Hit
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG58650.10261131359277113No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA158800.046.086561
AACGCAG191250.037.6625486
TCAACGC197050.036.5237964
CAACGCA200900.035.7350465
ATCAACG202100.035.522863
GTACATG289050.033.3810731
ACGCAGA220750.032.6833887
CATGGGT91200.032.5441324
GGTATCA94250.032.5018581
ACATGGG286450.032.4131363
TACATGG295350.032.2431372
CGCAGAG227550.031.7328348
TATCAAC231800.031.2682062
GCAGAGT253600.028.5201199
CATGGGG127850.027.9137064
AGAGTAC239100.024.44105710-11
ATGGGTA48200.022.4590475
ATGGGTG51950.021.1813265
CAGAGTA251300.020.65094610-11
GAGTACT81250.020.24131612-13
Produced by FastQC (version 0.11.5)