FastQCFastQC Report
Fri 10 Feb 2017
ERR1147339.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1147339.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4946933
Sequences flagged as poor quality0
Sequence length125
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT87990.17786778191659358No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA107700.048.9792861
TCAACGC135100.038.3464244
GGTATCA62050.037.9437451
AACGCAG139450.037.150256
CAACGCA139750.037.0704965
ATCAACG145250.035.7896463
GTACATG221450.034.66771
ACATGGG221150.033.6997153
TACATGG231500.032.856842
ACGCAGA164000.031.5890377
TATCAAC169900.031.2370622
CATGGGT56850.029.7132364
CGCAGAG174800.029.6713398
CATGGGG113300.028.1907394
GCAGAGT195900.026.5969479
ATGGGGG59800.025.064645
ATGGGTA35400.024.3627785
AGAGTAC178600.023.8614510-11
GTAAGTA23900.022.6792852
ATGGGAG43850.022.3808425
Produced by FastQC (version 0.11.5)