FastQCFastQC Report
Fri 10 Feb 2017
ERR1147338.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1147338.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1607223
Sequences flagged as poor quality0
Sequence length125
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT61330.3815898602745232No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT40780.2537295695743528No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT33600.2090562417287458No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTTCATTAAT18050.11230551080963873No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA53150.051.57671
GGTATCA29900.050.6245881
AACGCAG67350.040.5355036
TCAACGC69200.039.3658684
CAACGCA71050.038.4245765
ATCAACG72300.037.6779863
TATCAAC79050.034.7328872
ACGCAGA79950.034.221567
GTACATG86050.032.826641
CGCAGAG83800.032.720318
TACATGG89050.031.8357352
ATGGGGG27900.031.1249855
ACATGGG89400.031.06993
GCAGAGT89000.030.942229
CATGGGG55050.029.3881384
CATACGT1107.768923E-427.0357114
GCGTACA1107.768923E-427.0357119
TGGGGGG22950.026.9532496
AGAGTAC83400.026.4586910-11
TATCACG1200.00128076224.8159312
Produced by FastQC (version 0.11.5)