FastQCFastQC Report
Fri 10 Feb 2017
ERR1147337.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1147337.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1139117
Sequences flagged as poor quality0
Sequence length125
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT41800.36695089266510816No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT26200.2300027126274123No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT23130.20305201309435292No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA37750.043.0880051
GGTATCA23450.042.9392621
TACCGTA600.001310169339.6564257
AACGCAG44150.036.6473856
TCAACGC46650.034.555924
ATCAACG47800.033.7245523
CAACGCA47900.033.654155
GTACATG54950.031.769521
ACGCAGA53200.030.4131987
TACATGG56850.029.9543552
TATCAAC54850.029.7439272
ACATGGG56650.029.2960513
CGCAGAG55950.028.9183568
CATGGGT14450.027.581114
GCAGAGT59700.027.101889
CATGGGG28900.026.3461364
GTACCGT1451.3424158E-424.6143346
GGTACCT7250.024.6143328
TAGGACA3650.024.4457444
TATGTAA4300.023.5399132
Produced by FastQC (version 0.11.5)