FastQCFastQC Report
Fri 10 Feb 2017
ERR1147336.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1147336.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1774359
Sequences flagged as poor quality0
Sequence length125
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT68430.38566039905115035No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT39940.22509537246972006No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT38460.21675433212782758No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTTCATTAAT18440.10392485398952524No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA54450.051.3634571
GGTATCA30600.046.965061
AACGCAG67100.040.8653146
TCAACGC68550.040.1744544
CAACGCA70500.038.9788785
ATCAACG71000.038.9557043
TATAACG1252.9469447E-838.1235432
GTACATG88600.034.998461
TACATGG89900.034.455372
ACGCAGA80450.033.936197
TATCAAC82050.033.9040532
ACATGGG89850.033.828233
CGCAGAG84250.032.5467388
CATGGGG52350.030.7913724
GCAGAGT92050.029.8534589
ATGGGGG27250.028.8126965
TAACGCA1704.43948E-727.9909444
AGAGTAC86000.025.76346610-11
TGGGGGG24000.024.7836466
CATGGGT22200.024.113824
Produced by FastQC (version 0.11.5)