FastQCFastQC Report
Fri 10 Feb 2017
ERR1147335.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1147335.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2498474
Sequences flagged as poor quality0
Sequence length125
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT98470.39412057119665844No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT59080.23646433783181256No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT54920.21981417457215885No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTTCATTAAT26430.10578457090207863No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA76300.052.9431271
GGTATCA42100.047.8342551
AACGCAG95500.042.1606036
TCAACGC99100.040.2689554
CAACGCA101200.039.4333345
ATCAACG102550.039.0302053
ACGCAGA112200.035.832357
TATCAAC116800.034.7160382
CGCAGAG118000.034.2727058
GTACATG117600.033.7420351
TACATGG123350.032.196792
ACATGGG122450.031.7643833
GCAGAGT128100.031.5704869
CATGGGT34000.028.3372424
CATGGGG63700.027.262454
AGAGTAC122100.026.74103410-11
CAGAGTA125550.024.22983210-11
GAGTACT61850.024.13545412-13
ATGGGGG28950.024.035815
AGTACTT66800.023.50438912-13
Produced by FastQC (version 0.11.5)