FastQCFastQC Report
Fri 10 Feb 2017
ERR1147334.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1147334.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3272613
Sequences flagged as poor quality0
Sequence length125
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT120140.3671072626063638No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT67100.205034936914325No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT62770.19180391937574043No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTTCATTAAT33820.10334249726441838No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA92000.058.105841
GGTATCA45950.052.916341
AACGCAG122800.042.9619146
TCAACGC123500.042.6702424
ATCAACG126900.041.573863
CAACGCA127450.041.3011175
TATCAAC141750.037.6024442
ACGCAGA144950.036.3558127
CGCAGAG150200.035.1642538
GTACATG157050.033.5071831
TACATGG161550.032.2564932
GCAGAGT168400.031.7523579
ACATGGG163050.031.6998583
CATGGGT45750.028.2115524
AGAGTAC156100.027.11005210-11
CATGGGG87550.025.6799414
GAGTACT75650.023.8620712-13
CAGAGTA163450.023.74400510-11
AGTACTT82050.023.74056612-13
ATGGGGG41850.021.1762565
Produced by FastQC (version 0.11.5)