FastQCFastQC Report
Fri 10 Feb 2017
ERR1147333.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1147333.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2266434
Sequences flagged as poor quality0
Sequence length125
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT75730.3341372393813365No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT43720.19290215377990272No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT40510.17873893526129594No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA60300.054.565781
GGTATCA31700.048.3250661
AACGCAG78650.041.972276
TCAACGC79850.040.894574
ATCAACG83400.039.2251743
CAACGCA85150.038.4888735
ACGCAGA92000.035.8170787
CGCAGAG95600.034.40618
TATCAAC96800.034.0899122
GTACATG103900.033.6760521
TACATGG108300.031.6800232
ACATGGG107450.031.6080683
GCAGAGT104650.031.487549
AGAGTAC99400.027.49586310-11
CATGGGT27650.026.8895234
CATGGGG57300.026.2623834
ATGGGGG28350.023.707935
CAGAGTA103300.023.26211710-11
AGTACTT54350.022.2706612-13
GAGTACT48450.021.9749312-13
Produced by FastQC (version 0.11.5)