FastQCFastQC Report
Fri 10 Feb 2017
ERR1147332.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1147332.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1865256
Sequences flagged as poor quality0
Sequence length125
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT95980.514567437391972No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT57630.30896563259949306No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT53040.2843577503570555No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA72750.049.155051
GGTATCA41600.045.846541
AACGCAG90050.039.1683436
TCAACGC95050.036.9827844
CAACGCA96950.036.3807075
ATCAACG97500.036.175483
GTACATG108550.035.2496721
TATCAAC102550.034.4992072
TACATGG114000.033.3852272
ACATGGG112700.033.301953
ACGCAGA106150.033.1715627
CATGGGG61950.033.123944
ATGGGGG33100.032.8842055
CGCAGAG107950.032.7286458
GCAGAGT115900.030.5863089
GGGTTAG8650.028.2499471
TGGGGGG26750.027.3492586
AGAGTAC113400.026.14666710-11
TTAGCGC1150.001007112725.860474
GTTAGAG10950.024.9866453
Produced by FastQC (version 0.11.5)