FastQCFastQC Report
Fri 10 Feb 2017
ERR1147331.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1147331.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2535983
Sequences flagged as poor quality0
Sequence length125
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT112100.4420376635016875No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT66340.2615948135299014No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT62570.24672878327654407No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA85700.055.3628351
GGTATCA45850.046.800361
AACGCAG109350.043.517696
TCAACGC112950.041.762034
ATCAACG114450.041.2666633
CAACGCA116350.040.7461475
TATCAAC126500.037.5712242
ACGCAGA128700.036.974827
CGCAGAG133350.035.730098
GTACATG129250.033.3882831
GCAGAGT145100.032.9187059
TACATGG133600.032.2756582
ACATGGG135150.031.1610343
CATGGGG68450.027.894994
AGAGTAC136600.027.65143410-11
CATGGGT34700.026.7417434
GGGTTAG7200.026.4913181
CAGAGTA141850.024.51036810-11
GAGTACT69700.024.27974312-13
TATACCG2951.8189894E-1024.1965735
Produced by FastQC (version 0.11.5)