FastQCFastQC Report
Fri 10 Feb 2017
ERR1147330.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1147330.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences731057
Sequences flagged as poor quality0
Sequence length125
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT22140.30284916224042724No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT17040.23308716009832336No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT11940.16332515795621955No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA11550.051.5970881
GTATCAA19050.044.4222451
AACGCAG24400.034.3719336
TCAACGC25250.032.508164
CAACGCA25550.032.1264575
GTACATG29500.031.7164651
ATCAACG26150.031.3893283
TACATGG30400.031.1460862
ACATGGG31150.029.9789413
ACGCAGA29850.028.494857
TATCAAC30100.027.6975542
CTAGACA2154.0436134E-927.6653524
CATGGGG15800.026.7285844
CGCAGAG31850.026.7055348
ATACGGC1150.001006384325.8610926
GCAGAGT34500.024.9990549
CTAGCAC2352.9148032E-722.7797683
AGAGTAC32800.022.48653210-11
CATGGGT7700.022.401754
GAGTACT17700.022.34719512-13
Produced by FastQC (version 0.11.5)