FastQCFastQC Report
Fri 10 Feb 2017
ERR1147329.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1147329.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences743333
Sequences flagged as poor quality0
Sequence length125
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT30920.4159643120916197No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT23120.3110315296105514No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT18100.24349786703940224No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA20550.046.0647281
GTATCAA32700.042.239961
GGGTTAG2000.041.675541
TAACGTT600.001310332139.6536187
AACGCAG38100.036.219056
TCAACGC41650.033.1319544
ATCAACG42300.032.482223
CAACGCA43350.031.832665
ACGCAGA45100.030.7293577
CGCAGAG45850.030.3564248
GTACATG41250.030.1651571
TATCAAC47350.029.408612
TACATGG42350.029.3678052
ACATGGG43000.028.9102523
GCAGAGT48550.028.5457029
CATGGGT10200.025.6582244
CATGGGG22300.025.3391954
AGAGTAC46700.025.15499910-11
AGCGTCA1901.1706416E-625.044393
GGTTAGA2451.616354E-824.2891622
Produced by FastQC (version 0.11.5)