FastQCFastQC Report
Fri 10 Feb 2017
ERR1147328.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1147328.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1082723
Sequences flagged as poor quality0
Sequence length125
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT43960.4060133570636257No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT27140.25066429733181983No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT24760.2286826824589484No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTTCATTAAT13810.1275487820984684No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTAGGAATAATGGAA12650.11683505384110249No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGCAATACGAATGCC12240.11304830506048177No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAGGCCATGAT11940.11027751326978369No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA39400.048.0922971
AACGCAG45250.041.797496
GGTATCA24100.041.5371671
CAACGCA51150.036.7437175
TCAACGC51250.036.672024
ATCAACG51750.036.2027743
ACGCAGA53600.035.06427
GTACATG55350.034.4490281
CGCAGAG55900.033.8342828
TACATGG56750.033.0543172
ACATGGG56350.032.4030653
TATCAAC59350.032.007632
GCAGAGT59900.031.4756059
GTATAGG2700.030.8964731
CATGGGT16900.029.5620274
AGAGTAC56700.026.4862210-11
CATGGGG29850.026.300944
ATGGGTA7650.025.6563075
CAGAGTA59050.024.32421910-11
TATAACG1250.001625460323.8200952
Produced by FastQC (version 0.11.5)