FastQCFastQC Report
Fri 10 Feb 2017
ERR1147326.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1147326.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2087470
Sequences flagged as poor quality0
Sequence length125
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT100760.48268957158665754No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT59770.2863274681791834No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT57010.27310572127982674No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA81200.047.712331
GGTATCA47950.045.4952161
AACGCAG98400.039.414056
TCAACGC107050.035.8402984
ATCAACG108750.035.2800373
CAACGCA109150.035.2052465
ACGCAGA116650.033.1966937
GTACATG115800.032.5813031
TATCAAC119700.032.4930272
CGCAGAG119700.032.3011368
TACATGG120650.031.7434942
GCAGAGT128950.030.2608479
ACATGGG123750.029.946183
TATCACG1505.254191E-627.7957152
CATGGGG63300.026.969744
AGAGTAC123950.025.86668810-11
TAACGCA1859.2870505E-725.722724
GTATAGG4300.024.950391
CGTATAC1200.001290262824.7849083
ATGGGGG24600.024.4222035
Produced by FastQC (version 0.11.5)