FastQCFastQC Report
Fri 10 Feb 2017
ERR1147325.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1147325.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1396350
Sequences flagged as poor quality0
Sequence length125
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT62100.44473090557524975No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT37810.2707773838937229No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT35430.25373294661080675No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTTCATTAAT17820.12761843377376733No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAGGCCATGAT15240.10914169083682458No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGCAATACGAATGCC15000.1074229240519927No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTAGGAATAATGGAA14280.10226662369749705No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA48500.054.207971
GGTATCA26250.051.0998421
TATAACG1256.148184E-1042.8976942
AACGCAG62500.041.968996
TCAACGC65400.039.7441834
TATCACG758.124868E-539.7200852
ATCAACG66250.039.1444783
CAACGCA67000.039.0613985
TATCAAC72950.036.0175972
ACGCAGA73900.035.4947477
GTACATG79050.034.9931031
CGCAGAG76150.034.4459848
TACATGG80700.033.8875852
ACATGGG80650.033.261523
CATGGGT24400.031.6900884
GCAGAGT83050.031.6557469
TAACGCA1751.677472E-830.589644
ATGGGGG14450.029.2253995
ATGGGTA10600.028.0565385
TAGGACG850.007222819627.9905224
Produced by FastQC (version 0.11.5)